Ikhithi Yokuqoqwa kwamathe ye-J.able Bio

Umqoqi wamathe ubizwa nangokuthi isampula yamathe, idivayisi yokuqoqa amathe, ishubhu lokuqoqa amathe le-DNA.

Amathe ayingxube eyinkimbinkimbi engaqukethe amaprotheni ahlukahlukene kuphela, kodwa futhi i-DNA, i-RNA, ama-fatty acids, nama-microorganisms ahlukahlukene.Ucwaningo luye lwathola ukuthi izingxenye ezihlukahlukene zamaprotheni egazini zikhona futhi ematheni, futhi amathe angabonisa izinguquko emazingeni amaprotheni ahlukahlukene egazini.Ngakho-ke, kungenzeka ukuxilonga izifo ngokuthola amathe.

Ngokuthuthuka komphakathi kanye nokwenza ngcono okuqhubekayo kwamazinga okuphila amasiko, abantu banezidingo eziphakeme neziphakeme zokuhlolwa kwezokwelapha, ezidinga izindlela zokuhlola ezingahlaseli, ezilula, nezisheshayo kanye nezindlela zokuxilonga.Uma kuqhathaniswa nezibonelo ze-serum, ukuqoqwa kwamathe kuphephile futhi kulula, akuhlaseli, ngaphandle kwengozi yokusabalala kwezifo ezibangelwa igazi, akubuhlungu ezigulini, futhi kulula ukwamukela.Uma kuqhathaniswa namasampula omchamo, amathe anenzuzo yesampula yesikhathi sangempela.Ucwaningo lokutholwa kwamathe luvuse isasasa elikhulu futhi eminye imiphumela yokuqala izuziwe.Ukutholwa kwamathe sekusetshenziswe kakhulu.

Ukuhlolwa kwamathe kungenziwa ezindaweni ezihlukahlukene, kuhlanganise nasohlangothini lomgwaqo, futhi kunikeze ulwazi olubalulekile lokuxilonga.Amathe asetshenziselwe i-HIV, igciwane le-HBV, nezidakamizwa ezihlukahlukene ezifana ne-cocaine, ukuhlolwa kotshwala, ukuhlolwa kofuzo, ukuhlolwa kwegciwane, njll.

Incazelo yomkhiqizo wekhithi yokuqoqwa kwamathe ye-J.able:

Ikhithi yokuqoqa amathe yenziwe ifaneli, ishubhu lesampula, nesixazululo sokulondoloza.Ifaneli ingathumela amathe ngqo ku-buffer ezinzile engenabuthi.Kukhona imigqa ecacile yokuthweswa iziqu ebusweni bomqoqi wamathe.Kulula kakhulu ukuthutha isampula kanye nesitoreji.

Umqoqi wamathe ungasetshenziswa ukuqoqa amasampula amathe akhishelwe umgodi womlomo, futhi axube ngokulinganayo amathe aqoqiwe noketshezi lokulondoloza amathe, kuqinisekiswe ubuqotho be-DNA kusampula yamathe kanye nokugcinwa kwesikhathi eside ekamelweni lokushisa.Ngemuva kokukhishwa kwesampula, isetshenziselwa ukuxilongwa kwe-in vitro emtholampilo.

Indlela yokuqoqa:

1. Khafulela ngobumnene u-2ml wamathe eqongeni lamathe.

2. Thela ikhambi lokulondoloza ematheni bese uqinisa isivalo.

3. Gxumela phezulu naphansi ukuze uhlanganise amathe noketshezi lokulondoloza ngokulinganayo.

4. Faka ishubhu lesampula esikhwameni se-biosafety ukuze lihlolwe.

5. Lahla ifaneli.

Qaphela: Ngoba i-DNA ayikhishwa ematheni ngokwawo, kodwa kumaseli akhishwe ematheni.Ngakho-ke, lapho uqoqa amasampula amathe, sicela uzame ukusebenzisa ulimi lwakho ukunwaya umhlathi ongenhla nowaphansi kaningi ngangokunokwenzeka, futhi usebenzise amazinyo akho ukunwaya ulimi kancane ukuze uqinisekise inani lamaseli akhishwe.Ngakho-ke, ungadli, uphuze, ungabhemi, njll. ngaphambi kokuthatha isampula.


Isikhathi sokuthumela: Jun-25-2021